-
A rotavirus vaccine candidate attenuated by codon deoptimization protects neonatal mice against wild-type virus infection.Chen, Z., Kotaki, T., Motooka, D., Sato, S., Sakai, Y., Hirai, K., Minami, S., Kawagishi, T., Kanai, Y., & Kobayashi, T. (2026). PLoS pathogens, 22(7), e1014292, 2026.
- Recovery of Infectious Recombinant Human Norovirus Using Zebrafish Embryos.
Kotaki T, Akieda Y, Chen Z, Onishi M, Komatsu S, Motooka D, Omori H, Tamiya S, Kanai Y, Minami S, Kawagishi T, Sakon N, Sato S, Ishitani T, Kobayashi T.
PNAS, 122(49):e2526726122, 2025. - Single-round infectious rotaviruses with deletions of VP7 or VP4 genes, based on SA11 and WC3 strain backbones, and their potential use as viral vectors.
Kotaki T, Kanai Y, Onishi M, Sakai Y, Chen Z, Enoki Y, Komatsu S, Hirai K, Minami S, Kawagishi T, Ushijima H, Kobayashi T.
PLOS Pathogens, 21(9):e1013484. 2025. - A rotavirus VP4 or VP7 monoreassortant panel identifies genotypes that are less susceptible to neutralization by systemic antibodies induced by vaccination or natural infection.
Kotaki T, Kanai Y, Ogden KM, Onishi M, Kumthip K, Khamrin P, Boonyos P, Phoosangwalthong P, Singchai P, Luechakham T, Minami S, Chen Z, Hirai K, Tacharoenmuang R, Mizushima H, Ushijima H, Maneekarn N, Kobayashi T.
mBio. 30:e0089725. 2025. - Establishment of reverse genetics systems for Colorado tick fever virus.
Minami S, Nouda R, Hirai K, Chen Z, Kotaki T, Kanai Y, Kobayashi T.
PLOS Pathog. 21(2):e1012921. 2025.
- Rapid production of recombinant rotaviruses by overexpression of NSP2 and NSP5 genes with modified nucleotide sequences.
Kanai Y, Kotaki T, Sakai S, Ishisaka T, Matsuo K, Yoshida Y, Hirai K, Minami S, Kobayashi T.
J Virol. e0099624. 2024. - Generation of single-round infectious rotavirus with a mutation in the intermediate capsid protein VP6.
Kotaki T, Kanai Y, Onishi M, Minami S, Chen Z, Nouda R, Nurdin JA, Yamasaki M, Kobayashi T.
J Virol. e0076224. 2024. - Vero cell-adapted SARS-CoV-2 strain shows increased viral growth through furin-mediated efficient spike cleavage.
Minami S, Kotaki T, Sakai Y, Okamura S, Torii S, Ono C, Motooka D, Hamajima R, Nouda R, Nurdin JA, Yamasaki M, Kanai Y, Ebina H, Maeda Y, Okamoto T, Tachibana T, Matsuura Y, Kobayashi T.
Microbiology spectrum, e0285923, 2024.
- Genetic engineering strategy for generating a stable dsRNA virus vector using a virus-like codon-modified transgene.
Kanai Y, Onishi M, Yoshida Y, Kotaki T, Minami S, Nouda R, Yamasaki M, Enoki Y, Kobayashi T.
Journal of virology, e0049223, 2023. - Efficient Selective Adsorption of SARS-CoV-2 via the Recognition of Spike Proteins Using an Affinity Spongy Monolith.
Kubo T, Kanao E, Ishida K, Minami S, Tanigawa T, Mizuta R, Sasaki Y, Otsuka K, Kobayashi T.
Analytical chemistry, (95) 35:13185-13190, 2023. - Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.
Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, J. A. Nurdin, Yamasaki M , Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.
Scientific reports, 2023. - Genotype Diversity of Enteric Viruses in Wastewater Amid the COVID-19 Pandemic.
S. A. Hoque, Kotaki T, N. T. K. Pham, Onda Y, Okitsu S, Sato S, Yuki Y, Kobayashi T, N. M. Maneekarn, Kiyono H, Hayakawa S, Ushijima H.
Food and environmental virology, (15)2:176-191, 2023. - Abundance of viral gastroenteritis before and after the emergence of COVID-19: Molecular evidence on wastewater.
S. A. Hoque, Kotaki T, N. T. K. Pham, Onda Y, Okitsu S, Sato S, Yuki Y, Kobayashi T, N. M. Maneekarn, Kiyono H, Hayakawa S, Ushijima H.
Journal of infection, (86)2:154-225, 2023. - N-glycosylation of rotavirus NSP4 protein affects viral replication and pathogenesis.
Jeffery A. N., Kotaki T, Kawagishi T, Sato S, Yamasaki M, Nouda R, Minami S, Kanai Y, Kobayashi T.
Journal of Virology, e01861-22, 2023. - Characterization of Sialic Acid-Independent Simian Rotavirus Mutants in Viral Infection and Pathogenesis.
Yamasaki M, Kanai Y, Wakamura Y, Kotaki T, Minami S, Nouda R, Jeffery A. N., Kobayashi T.
Journal of Virology, e01397-22, 2023. - Functional changes in cytotoxic CD8+ T-cell cross-reactivity against the SARS-CoV-2 Omicron variant after mRNA vaccination.
Nogimori T, Suzuki K, Masuta Y, Washizaki A, Yagoto M, Ikeda M, Katayama Y, Kanda H, Takada M, Minami S, Kobayashi T, Takahama S, Yoshioka Y, Yamamoto T.
Front Immunol. 4;13:1081047. 2023.
- A reverse genetics system for human rotavirus G2P[4].
Hamajima R, Tina L., Minami S, Nouda R, Jeffery A. N., Yamasaki M, Kobayashi N, Kanai Y, Kobayashi T.
Journal of General Virology, 103(12), 2022. - Cell response analysis in SARS-CoV-2 infected bronchial organoids.
Sano E, Suzuki T, Hashimoto R, Itoh Y, Sakamoto A, Sakai Y, Saito A, Okuzaki D, Motooka D, Muramoto Y, Noda T, Takasaki T, Sakuragi J, Minami S, Kobayashi T, Yamamoto T, Matsumura Y, Nagao M, Okamoto T, Takayama K.
Commun Biol. 5 (1) : 516, 2022. - Development of an Oncolytic Mammalian Orthoreovirus Expressing the Near-Infrared Fluorescent Protein iRFP720.
Ogawa K, Yamada K, Etoh T, Kitagawa M, Shirasaka Y, Noguchi K, Kobayashi T, Nishizono A, Inomata M.
Journal of Virological Methods, 308:114574, 2022. - Emerging norovirus GII.4 Sydney[P31] causing acute gastroenteritis outbreak in children in Japan, during COVID-19, 2021.
Ngan T. K. P., Nishimura S, Shimizu-Onda Y, Quang D. T., Komine-Aizawa S , Pattara K., Okitsu S , Sato S, Kobayashi T, Niwat M., Hayakawa S, Ushijima H.
J Infect Chemother, 28(9):1347-1351, 2022. - Changing Predominance of Norovirus Recombinant Strains GII.2[P16] to GII.4[P16] and GII.4[P31] in Thailand, 2017 to 2018.
Pattara K., Kattareeya K. , Arpaporn Y. , Nutthawadee J. ,Phitchakorn P. , Panuwat Y, Okitsu S , Kobayashi T, Ushijima H, Niwat M.
Microbiology spectrum, 10(3) e00448-22, 2022. - Whole genome sequencing and evolutionary analysis of G8P [8] rotaviruses emerging in Japan.
Tung P., Kobayashi M, Nagasawa , Hatazawa R, Ngan T. K. P., Miyashita H, Komoto S, Tajima T, Baba T, Okitsu S, Pattara K., Niwat M., Kimura H, Kobayashi T, Hayakawa S, Ushijima H.
Virusdisease, 33(2):215-218,2022. - The nonstructural p17 protein of a fusogenic bat-borne reovirus regulates viral replication in virus species- and host-specific manners.
Nouda R, Kawagishi T, Kanai Y, Shimojima M, Saijo M, Matsuura Y, Kobayashi T.
PLOS Pathogens 18(6): e1010553, 2022. - SARS-CoV-2 infection triggers paracrine senescence and leads to a sustained senescence-associated inflammatory response.
Tsuji S, Minami S, Hashimoto R, Konishi Y, Suzuki T, Kondo T, Sasai M, Torii S, Ono C, Shichinohe S, Sato S, Wakita M, Okumura S, Nakano S, Matsudaira T, Matsumoto T, Kawamoto S, Yamamoto M, Watanabe T, Matsuura Y, Takayama K, Kobayashi T, Okamoto T, Hara E.
Nat Aging. 2(2):115-124. 2022.
- Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detection.
Taniguchi M, Minami S, Ono C, Hamajima R, Morimura A, Hamaguchi S, Akeda Y, Kanai Y, Kobayashi T, Kamitani W, Terada Y, Suzuki K, Hatori N, Yamagishi Y, Washizu N, Takei H, Sakamoto O, Naono N, Tatematsu K, Washio T, Matsuura Y, Tomono K.
Nat Commun. 17;12(1):3726. 2021. - Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses.
Nouda R, Minami S, Kanai Y, Kawagishi T, Nurdin JA, Yamasaki M, Kuwata R, Shimoda H, Maeda K, Kobayashi T.
Proc Natl Acad Sci U S A. 118(42): e2105334118. 2021. - Monoreassortant rotaviruses of multiple G types are differentially neutralized by sera from infants vaccinated with ROTARIXR and RotaTeqR.
Diller JR, Carter MH, Kanai Y, Sanchez SV, Kobayashi T, Ogden KM.
J Infect Dis. jiab479. 2021. - FAST Proteins: Development and Use of Reverse Genetics Systems for Reoviridae Viruses.
Kanai Y, Kobayashi T.
Annu Rev Virol. 8(1): 515-536. 2021. - Epidemiology and genetic diversity of group A rotaviruses in pre-vaccination period in Thailand, 2018-2019.
Jampanil N, Kumthip K, Yodmeeklin A, Kanai Y, Okitsu S, Kobayashi T, Ukarapol N, Ushijima H, Maneekarn N, Khamrin P.
Infect Genet Evol.104898. 2021. - Rotavirus reverse genetics systems: Development and application.
Kanai Y, Kobayashi T.
Virus Res. 295:198296. 2021. - Generation of recombinant rotaviruses encoding a split NanoLuc peptide tag.
Pannacha P, Kanai Y, Kawagishi T, Nouda R, Nurdin JA, Yamasaki M, Nomura K, Lusiany T, Kobayashi T.
Biochem Biophys Res Commun. 534:740-746. 2021. - Reverse Genetics Approach for Developing Rotavirus Vaccine Candidates Carrying VP4 and VP7 Genes Cloned from Clinical Isolates of Human Rotavirus.
Kanai Y, Onishi M, Kawagishi T, Pannacha P, Nurdin JA, Nouda R, Yamasaki M, Lusiany T, Khamrin P, Okitsu S, Hayakawa S, Ebina H, UshijimaH, Kobayashi T.
J Virol. 95(2):e01374-20. 2021. - Construction and characterization of an infectious clone generated from Chikungunya virus SL11131 strain.
Suzuki Y, Tanaka A, Maeda Y, Emi A, Fujioka Y, Sakaguchi S, Vasudevan SG, Kobayashi T, Lim CK, Takasaki T, Wu H, Nakano T.
Virology. 552:52-62. 2021.
- Generation of Genetically RGD σ1-Modified Oncolytic Reovirus That Enhances JAM-A-Independent Infection of Tumor Cells.
Kawagishi T, Kanai Y, Nouda R, Fukui I, Nurdin JA, Matsuura Y, Kobayashi T.
J Virol 94(23):e01703-20. - Reverse Genetics System for a Human Group A Rotavirus.
Kawagishi T, Nurdin JA, Onishi M, Nouda R, Kanai Y, Tajima T, Ushijima H, Kobayashi T.
J Virol 94(2):e00963-19.
- In Vivo Live Imaging of Oncolytic Mammalian Orthoreovirus Expressing NanoLuc Luciferase in Tumor Xenograft Mice.
Kanai Y, Kawagishi T, Matsuura Y, Kobayashi T.
J Virol 93:e00401-19. - Cell-cell fusion induced by reovirus FAST proteins enhances replication and pathogenicity of non-enveloped dsRNA viruses.
Kanai Y, Kawagishi T, Sakai Y, Nouda R, Shimojima M, Saijo M, Matsuura Y, Kobayashi T.
PLoS Pathog 15(4): e1007675.
- Development of stable rotavirus reporter expression systems.
Kanai Y, Kawagishi T, Nouda R, Onishi M, Pannacha P, Nurdin JA, Nomura K, Matsuura Y, Kobayashi T.
J Virol 93: e01774-18.
- Lethal murine infection model for human respiratory disease-associated Pteropine orthoreovirus.
Kanai Y, Kawagishi T, Okamoto M, Sakai Y, Matsuura Y, Kobayashi T.
Virology 514:57-65. - Reverse genetics system demonstrates that rotavirus nonstructural protein NSP6 is not essential for viral replication in cell culture.
Komoto S, Kanai Y, Fukuda S, Kugita M, Kawagishi T, Ito N, Sugiyama M, Matsuura Y, Kobayashi T, Taniguchi K.
J Virol 91(21): e00695-17. - Entirely plasmid-based reverse genetics system for rotaviruses.
Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, Matsuura Y, Taniguchi K, Kobayashi T.
Proc Natl Acad Sci U S A 114:2349-2354. - African swine fever virus NP868R capping enzyme promotes reovirus rescue during reverse genetics by promoting reovirus protein expression, virion assembly, and RNA incorporation into infectious virions.
Eaton HE, Kobayashi T, Dermody TS, Johnston RN, Jais PH, Shmulevitz M.
J Virol 91(11): e02416-16.
- Reverse genetics for fusogenic bat-borne orthoreovirus associated with acute respiratory tract infections in humans: role of outer capsid protein σC in viral replication and pathogenesis.
Kawagishi T, Kanai Y, Tani H, Shimojima M, Saijo M, Matsuura Y, Kobayashi T.
PLoS Pathog 12:e1005455. - Novel antiviral activity of bromocriptine against dengue virus replication.
Kato F, Ishida Y, Oishi S, Fujii N, Watanabe S, Vasudevan SG, Tajima S, Takasaki T, Suzuki Y, Ichiyama K, Yamamoto N, Yoshii K, Takashima I, Kobayashi T, Miura T, Igarashi T, Hishiki T.
Antiviral Res 131:141-147.
- Rapid whole genome sequencing of Miyazaki-Bali/2007 Pteropine orthoreovirus by modified rolling circular amplification with adaptor ligation - next generation sequencing.
Singh H, Yoshikawa T, Kobayashi T, Fukushi S, Tani H, Taniguchi S, Fukuma A, Yang M, Sugamata M, Shimojima M, Saijo M.
Sci Rep 5:16517.
- Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus.
Yamanaka A, Iwakiri A, Yoshikawa T, Sakai K, Singh H, Himeji D, Kikuchi I, Ueda A, Yamamoto S, Miura M, Shioyama Y, Kawano K, Nagaishi T, Saito M, Minomo M, Iwamoto N, Hidaka Y, Sohma H, Kobayashi T, Kanai Y, Kawagishi T, Nagata N, Fukushi S, Mizutani T, Tani H, Taniguchi S, Fukuma A, Shimojima M, Kurane I, Kageyama T, Odagiri T, Saijo M, Morikawa S.
PLoS One 9:e92777. - A plasmid-based reverse genetics system for mammalian orthoreoviruses driven by a plasmid-encoded T7 RNA polymerase.
Komoto S, Kawagishi T, Kobayashi T, Ikizler M, Iskarpatyoti J, Dermody TS, Taniguchi K.
J Virol Methods 196:36-39. - Development of a novel Dengue-1 virus replicon system expressing secretory Gaussia luciferase for analysis of viral replication and discovery of antiviral drugs.
Kato F, Kobayashi T, Tajima S, Takasaki T, Miura T, Igarashi T, Hishiki T.
Jpn J Infect Dis 67:209-212. - Immunogenicity of recombinant VP2 proteins of all nine serotypes of African horse sickness virus.
Kanai Y, van Rijn PA, Maris-Veldhuis M, Kaname Y, Athmaram TN, Roy P.
Vaccine 32:4932-4937.
- Construction and characterization of an infectious molecular clone of Koala retrovirus.
Shojima T, Hoshino S, Abe M, Yasuda J, Shogen H, Kobayashi T, Miyazawa T.
J Virol 87:5081-5088. - Natural infection of cynomolgus monkeys with dengue virus occurs in epidemic cycles in the Philippines.
Kato F, Ishida Y, Kawagishi T, Kobayashi T, Hishiki T, Miura T, Igarashi T.
J Gen Virol 94:2202-2207. - Changes in gene expression associated with conceptus implantation to the maternal endometrium.
Imakawa K, Yasuda J, Kobayashi T, Miyazawa T.
Journal of Mammalian Ova Research 30:2-10. - Generation of a replication-competent chimeric simian-human immunodeficiency virus carrying env from subtype C clinical isolate through intracellular homologous recombination.
Fujita Y, Otsuki H, Watanabe Y, Yasui M, Kobayashi T, Miura T, Igarashi T.
Virology 436:100-111. - Nonstructural protein σ1s mediates reovirus-induced cell cycle arrest and apoptosis.
Boehme KW, Hammer K, Tollefson WC, Konopka-Anstadt JL, Kobayashi T, Dermody TS.
J Virol 87:12967-12979. - Recovery of African horse sickness virus from synthetic RNA.
Kaname Y, Celma CC, Kanai Y, Roy P.
J Gen Virol 94:2259-2265. - Multiple large foreign protein expression by a single recombinant baculovirus: a system for production of multivalent vaccines.
Kanai Y, Athmaram TN, Stewart M, Roy P.
Protein Expr Purif 91:77-84.
- Canine ASCT1 and ASCT2 are functional receptors for RD-114 virus in dogs.
Yoshikawa R, Yasuda J, Kobayashi T, Miyazawa T.
J Gen Virol 93:603-607. - Sequence comparison of three infectious molecular clones of RD-114 virus.
Shimode S, Yoshikawa R, Hoshino S, Nakaya Y, Sakaguchi S, Kobayashi T, Miyazawa T.
Virus Genes 45:393-397. - Identification and characterization of feline UBE1L gene.
Shimode S, Miyazawa T, Kobayashi T, Sato H, Tanabe T.
J Vet Med Sci 74:235-239. - Binding of transcription factor activating protein 2 gamma on the 5'-proximal promoter region of human porcine endogenous retrovirus subgroup A receptor 2/GPR172B.
Nakaya Y, Shimode S, Kobayashi T, Imakawa K, Miyazawa T.
Xenotransplantation 19:177-185. - Lymph nodes harbor viral reservoirs that cause rebound of plasma viremia in SIV-infected macaques upon cessation of combined antiretroviral therapy.
Horiike M, Iwami S, Kodama M, Sato A, Watanabe Y, Yasui M, Ishida Y, Kobayashi T, Miura T, Igarashi T.
Virology 423:107-118. - Reliability of a newly-developed immunochromatography diagnostic kit for pandemic influenza A/H1N1pdm virus: implications for drug administration.
Sasaki T, Kubota-Koketsu R, Takei M, Hagihara T, Iwamoto S, Murao T, Sawami K, Fukae D, Nakamura M, Nagata E, Kawakami A, Mitsubayashi Y, Ohno M, Uehara Y, Fukukawa T, Kanai Y, Kosaka M, Ikuta K.
PLoS One 7:e50670. - Hepatitis E virus in Norway rats (Rattus norvegicus) captured around a pig farm.
Kanai Y, Miyasaka S, Uyama S, Kawami S, Kato-Mori Y, Tsujikawa M, Yunoki M, Nishiyama S, Ikuta K, Hagiwara K.
BMC Res Notes. - Neutralization titers against influenza A (H3N2) and influenza B viruses among a non-vaccinated population from Thailand.
Boonsathorn N, Kanai Y, Punjumpa J, Bai G, Chittaganpitch M, Petphuwadee U, Jampangern W, Sawanpanyalert P, Ikuta K, Teerasut C.
Southeast Asian J Trop Med Public Health 43:674-679.
- Crystal structure of reovirus attachment protein σ1 in complex with sialylated oligosaccharides.
Reiter DM, Frierson JM, Halvorson EE, Kobayashi T, Dermody TS, Stehle T.
PLoS Pathog 7:e1002166. - Reverse genetics for mammalian reovirus.
Boehme KW, Ikizler M, Kobayashi T, Dermody TS.
Methods 55:109-113. - The reovirus sigma1s protein is a determinant of hematogenous but not neural virus dissemination in mice.
Boehme KW, Frierson JM, Konopka JL, Kobayashi T, Dermody TS.
J Virol 85:11781-11790.
- A post-entry step in the mammalian orthoreovirus replication cycle is a determinant of cell tropism.
Ooms LS, Kobayashi T, Dermody TS, Chappell JD.
J Biol Chem 285:41604-41613. - An improved reverse genetics system for mammalian orthoreoviruses.
Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS.
Virology 398:194-200. - Characterization of H5N1 influenza viruses isolated from humans in vitro.
Li YG, Chittaganpitch M, Waicharoen S, Kanai Y, Bai GR, Kameoka M, Takeda N, Ikuta K, Sawanpanyalert P.
Virol J 7:112. - Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies.
Kanai Y, Tsujikawa M, Yunoki M, Nishiyama S, Ikuta K, Hagiwara K.
J Med Virol 82:69-76. - Distinct propagation efficiencies of H5N1 influenza virus Thai isolates in newly established murine respiratory region-derived cell clones.
Kanai Y, Chittaganpitch M, Nakamura I, Li GM, Bai GR, Li YG, Ikuta K, Sawanpanyalert P.
Virus Res 153:218-225. - The impact of antigenic drift of influenza A virus on human herd immunity: Sero-epidemiological study of H1N1 in healthy Thai population in 2009.
Kanai Y, Boonsathorn N, Chittaganpitch M, Bai G, Li Y, Kase T, Takahashi K, Okuno Y, Jampangern W, Ikuta K, Sawanpanyalert P.
Vaccine 28:5437-5444.
- Reovirus mu2 protein inhibits interferon signaling through a novel mechanism involving nuclear accumulation of interferon regulatory factor 9.
Zurney J, Kobayashi T, Holm GH, Dermody TS, Sherry B.
J Virol 83:2178-2187. - Identification of functional domains in reovirus replication proteins muNS and mu2.
Kobayashi T, Ooms LS, Chappell JD, Dermody TS.
J Virol 83:2892-2906. - Amantadine- and oseltamivir-resistant variants of influenza A viruses in Thailand.
Bai GR, Chittaganpitch M, Kanai Y, Li YG, Auwanit W, Ikuta K, Sawanpanyalert P.
Biochem Biophys Res Commun 390:897-901.
- Reovirus apoptosis and virulence are regulated by host cell membrane penetration efficiency.
Danthi P, Kobayashi T, Holm GH, Hansberger MW, Abel TW, Dermody TS.
J Virol 82:161-172.
- A plasmid-based reverse genetics system for animal double-stranded RNA viruses.
Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, Wilson GJ, Chappell JD, Dermody TS.
Cell Host Microbe 1:147-157.
