Influenza Virus

Avian Influenza Virus

- We have some evidence indicating the emergence of sublineage in avian flu H5N1 in chickens and ducks in Egypt since 2008 by dynamic mutation at envelope region.

- Some of H1N1 isolats from Egypitian chikens after 2008 may acquire higher affinity to human respiratory regions.

- Some of H1N1 isolats from Egypitian chikens after 2008 may induce antigenic shift as evidenced by faith to be reacted with some monoclonal antibodies against viral envelope protein.

Seasonal Influenza Virus

- We are preparing several human neutralizing monoclonal antibodies by use of the peripheral blood mononuclear cells derived from healthy donor vaccinees. So far, we have obtained several neutralizing monoclonal antibodies against AH3N2, B, and swine-origin pandemic H1N1.

- Comparative evaluation of human neutralizing monoclonal antibodies and anti-flu drug (Tamiflu) are ongoing in mice.

- Our products of human neutralizing monoclonal antibodies are type-specific. Hwever, such antibodies can be reacted with wide range of viral strains within the same type. In fact, the recognized epitope to form anti-parallel beta sheet structure was found to be highly conservative and similar conformational structures were identified in all the type sof influenza virus. Therefore, we are currently collaborating with companies for the possiblity to develop the conformational epitope vaccine.

Swine-Origin Pandemic Influenza Virus

- For diagnosis of swine-origin pandemic H1N1, the immunochromato kits develped for seasonal flu have been used before RT-PCR. However the sensitivity in some of the kits seem to be lower in pandemic flu. Therefore, we prepared several murine monoclonal antibodes that reacted only with pandemic flu, but not seasonal flu viruses.

- By collaboration with several companies in Japan, we have developed new type of immunchromato kits with high sensitivity and high specificity to pandemic flu. The kits seem to be highly useful for the diagnostics in hospital as on-spot, and there is no necessity for PCR confiormation.